Expression of gonadotropin-inhibitory hormone receptors in mouse pituitary gonadotroph LβT2 cells and hypothalamic gonadotropin-releasing hormone-producing GT1-7 cells
نویسندگان
چکیده
منابع مشابه
Local Regulation of Gonadotroph Function by Pituitary Gonadotropin-Releasing Hormone.
Cultured rat pituitary cells and immortalized pituitary gonadotrophs (alphaT3-1 cells) express specific messenger RNA transcripts for GnRH and exhibit positive immunostaining for the GnRH peptide. Each cell type released GnRH during both static culture and perifusion, albeit in lesser amounts than cultured hypothalamic cells and GT1-7 neurons. In perifused pituitary cells, exposure to a GnRH ag...
متن کاملKisspeptin induces expression of gonadotropin-releasing hormone receptor in GnRH-producing GT1-7 cells overexpressing G protein-coupled receptor 54.
Kisspeptin signaling through its receptor is crucial for many reproductive functions. However, the molecular mechanisms and biomedical significance of the regulation of GnRH neurons by kisspeptin have not been adequately elucidated. In the present study, we found that kisspeptin increases GnRH receptor (GnRHR) expression in a GnRH-producing cell line (GT1-7). Because cellular activity of G prot...
متن کاملPost-transcriptional regulation of the gonadotropin-releasing hormone gene in GT1-7 cells.
GT1-7 cells respond to treatment with the phorbol ester, phorbol 12-myristate 13-acetate (PMA), with an inhibition of transcription of the proGnRH gene and decreases in GnRH mRNA levels. However, the timing of this decrease in GnRH mRNA levels suggests that a decrease in GnRH mRNA stability may be involved in addition to an inhibition of transcription of the proGnRH gene. To address this possib...
متن کاملInsulin-like growth factor-I effects on gonadotropin-releasing hormone biosynthesis in GT1-7 cells.
The immortalized GT1-7 cell line synthesizes and secretes GnRH, the key hormone of reproduction. However, GT1-7 cells lack the normal inputs from neurotransmitters, growth factors, and steroids, which are involved in the maturation and maintenance of GnRH neurons in the brain. We examined the effects of the neurotrophic factor insulin-like growth factor-I (IGF-I) on GnRH gene expression and the...
متن کاملP-95: Seasonal Changes in Hypothalamic Gonadotropin-Inhibitory Hormone Expression in Alectoris Chukar
Background Gonadotropin-inhibitory hormone (GnIH), initially discovered in birds as a hypothalamic neuropeptide, inhibits the synthesis and release of gonadotropins via affecting GnRH neurons and gonadotropes. Therefore, it could be a key neuropeptide in regulating seasonal breeding in birds. The aim of the present study was to investigate the expression of GnIH in the hypothalamus of male and ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Endocrine Journal
سال: 2014
ISSN: 0918-8959,1348-4540
DOI: 10.1507/endocrj.ej13-0238